Profile of Exosomes Related Proteins Released by Differentiated and Undifferentiated Human Keratinocytes

Our group has previously demonstrated the capacity of human keratinocytes to release 14-3-3 sigma into conditioned medium through the mechanism of exosome externalization. In this study the release of other proteins through the same mechanism and the differences in the profiles of 14-3-3 proteins between differentiated (diff-K) and undifferentiated keratinocytes (undiff-K) were investigated. The stimulatory effect of other 14-3-3 isoforms on the expression of MMP-I in dermal fibroblasts was also evaluated. Exosomes isolated from undiff-K (low Ca2+) and diff-K (high Ca2+) were subjected to proteomic and Western blot analysis. The results showed that more than 50 different cytoplasmic proteins including all seven 14-3-3 protein isoforms (beta, sigma, eta, epsilon, tau, zeta, and gamma) were released from diff-K through the mechanism of exosome externalization. However, in exosomes of undiff-K only four of the 14-3-3 protein isoforms (beta, eta, zeta, and gamma) were detected. Ca2+ treatment increased the release of exosomes from undiff-K by at least two times relative to the control. Consistent with this finding, the stimulatory effect of exosomes containing 14-3-3 sigma from diff-K had higher MMP-I stimulatory effect in fibroblasts relative to those exosomes isolated from undiff-K. MMP-I stimulatory effect of recombinant 14-3-3 beta and eta, tested in this study, in dermal fibroblasts, suggests additional anti-fibrogenic factors other than 14-3-3 sigma. In conclusion, keratinocytes release many proteins through the mechanism of exosome externalization from which some such as 14-3-3 isoforms may function as extracellular matrix (ECM) modulating factors for dermal fibroblasts. These findings revealed the presence of a novel mechanism by which keratinocytes can potentially interact with fibroblasts. J. Cell. Physiol. 221: 221-23 1, 2009. (C) 2009 Wiley-Liss, Inc.