Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 112, Issue 3, Pages 872-880Publisher
WILEY-BLACKWELL
DOI: 10.1002/jcb.22995
Keywords
CDK2AP1; miR-21; HNSCCS; INVASION; PROLIFERATION
Categories
Funding
- National Natural Science Foundation of China [30572059, 30772428]
- local Ethics Committee
- National Board of the Medical Affairs
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The present study was undertaken to investigate the regulation of P12(CDK2AP1) by miRNAs. A conserved target site for miR-21 within the CDK2AP1-3'-UTR at nt 349-370 was predicted by bioinfomatics software and an inverse correlation of miR-21 and CDK2AP1 protein was observed. Highly specific amplification and quantification of miR-21 was achieved using real-time RT-PCR. Transfection of HaCaT cells with pre-miR-21 significantly suppressed a luciferase reporter including the CDK2AP1-3'-UTR, whereas transfection of Tec8113 with anti-miR-21 increased activity of this reporter. This was abolished when a construct mutated at the miR-21/nt 349-370 target site was used instead. Anti-miR-21-transfected Tca8113 cells showed an increase of CDK2AP1 protein and reduced proliferation and invasion. Resected primary tumors and tumor-free surgical margins of 18 patients with head and neck squamous cell carcinomas demonstrated an inverse correlation between miR-21 and P12(CDK2AP1). This study shows that P12(CDK2AP1) is downregulated by miR-21 and that miR-21 promotes proliferation and invasion in cultured cells. J. Cell. Biochem. 112: 872-880, 2011. (C) 2010 Wiley-Liss, Inc.
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