Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 105, Issue 4, Pages 1073-1080Publisher
WILEY-BLACKWELL
DOI: 10.1002/jcb.21910
Keywords
Drak2; TYPE 2 DIABETES; ISLET
Categories
Funding
- Canadian Institutes of Health Research (CIHR) [MOP57697, MOP69089, PPP85159, MOP79565]
- Kidney Foundation of Canada
- Heart and Stroke Foundation of Quebec
- Juvenile Diabetes Research Foundation USA [1-2005-197]
- J.-Louis Levesque Foundation
- Fonds de la Recherche en Saute du Quebec for Transfusional and Hemovigilance Medical Research
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Drak2 is a serine threonine kinase in the death-associated protein family. In this study, we investigated its role in free Fatty acid (FFA)-induced islet apoptosis. Drak2 mRNA and protein were rapidly induced in islet P-cells after FFA stimulation. Such Drak2 upregulation was accompanied by increased P-cell apoptosis, which was inhibited by Drak2 knockdown using siRNA. Conversely, transgenic (Tg) Drak2 overexpression led to aggravated P-cell apoptosis triggered by FFA. Drak2 overexpression in islets compromised the increase of antiapoptotic factors, such as Bcl-2, Bcl-xL and Flip, upon FFA assault. Further in vivo experiments demonstrated that Drak2 Tg mice presented compromised glucose tolerance in a diet-induced obesity model. Our data show that Drak2 is detrimental to islet survival in the presence of excessive lipid. J. Cell. Biochem. 105: 1073-1080, 2008. (C) 2008 Wiley-Liss, Inc.
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