Journal
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 14, Issue 6B, Pages 1594-1604Publisher
WILEY
DOI: 10.1111/j.1582-4934.2009.00887.x
Keywords
SDF-1 alpha; ionizing radiation; tissue regeneration; MSCs; microenvironment
Categories
Funding
- Canadian Institute of Health Research (CIHR) [MOP-79317]
- Fondation de l'Hopital Sainte-Justine
- Health Research Foundation Rx&D (Health Research Foundation)/CIHR (Canadian Institute of Health Research (CIHR)
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Patients treated for cancer therapy using ionizing radiation (IR) have delayed tissue repair and regeneration. The mechanisms mediating these defects remain largely unknown at present, thus limiting the development of therapeutic approaches. Using a wound healing model, we here investigate the mechanisms by which IR exposure limits skin regeneration. Our data show that induction of the stromal cell-derived growth factor 1 alpha (SDF-1 alpha) is severely impaired in the wounded skin of irradiated, compared to non-irradiated, mice. Hence, we evaluated the potential of bone marrow-derived multipotent stromal cells (MSCs), which secrete high levels of SDF-1 alpha, to improve skin regeneration in irradiated mice. Injection of MSCs into the wound margin led to remarkable enhancement of skin healing in mice exposed to IR. Injection of irradiated MSCs into the wound periphery of non-irradiated mice delayed wound closure, also suggesting an important role for the stromal microenvironment in skin repair. The beneficial actions of MSCs were mainly paracrine, as the cells did not differentiate into keratinocytes. Specific knockdown of SDF-1 alpha expression led to drastically reduced efficiency of MSCs in improving wound closure, indicating that SDF-1 alpha secretion by MSCs is largely responsible for their beneficial action. We also found that one mechanism by which SDF-1 alpha enhances wound closure likely involves increased skin vascularization. Our findings collectively indicate that SDF-1 alpha is an important deregulated cytokine in irradiated wounded skin, and that the decline in tissue regeneration potential following IR can be reversed, given adequate microenvironmental support
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