Journal
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 12, Issue 5A, Pages 1767-1776Publisher
WILEY
DOI: 10.1111/j.1582-4934.2007.00190.x
Keywords
Pseudomonas; lung inflammation; caspases; Type III secretion; Interleukin-1
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Funding
- Interuniversitaire Attractiepolen [IAP6/18]
- Fonds voor Wetenschappelijk Onderzoek-Vlaanderen [grant 3G010505]
- King Baudouin Foundation (Alphonse and Jean Forton Fund)
- Ghent University [01G06B6]
- BOF of the Ghent University
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Pseudomonas aeruginosa is an opportunistic bacterial pathogen that forms a serious problem for immunocompromised patients and also the leading cause of mortality in cystic fibrosis. The overall importance of a functional Type III secretion system (T3SS) in P. aeruginosa virulence has been well established, but the underlying mechanisms are still unclear. Using in vitro infected macrophages as well as a murine model of acute lung infection, we show that the Caspase-1 mediated maturation and secretion of IL-1 beta needs a translocation competent T3SS and Flagellin, but not the Type III effector proteins ExoS, ExoT and ExoY. However, ExoS was found to negative regulate the P. aeruginosa induced IL-1 beta maturation by a mechanism that is dependent on its ADP ribosyltransferase activity. Moreover, ExoS deficiency also switched the mode of macrophage death from apoptosis to pro-inflammatory pyroptosis. Altogether, these data demonstrate a dual role for the P. aeruginosa T3SS in the regulation of Caspase-1 mediated IL-1 beta production and provide new insights into the mechanisms of immune evasion by this pathogen.
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