Journal
JOURNAL OF CELL SCIENCE
Volume 131, Issue 19, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.221481
Keywords
CKLF; CMTM gene cluster; CRISPR/Cas9; Fertilization; Testis; Zona pellucida
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT)/Japan Society for the Promotion of Science (JSPS) KAKENHI [JP15H05573, JP16KK0180, JP25112007, JP17H01394]
- Japan Agency for Medical Research and Development (AMED) [JP18gm5010001]
- Takeda Science Foundation
- Chubei Itoh Foundation
- Eunice Kennedy Shriver National Institute of Child Health and Human Development [R01HD088412, P01HD087157]
- Bill & Melinda Gates Foundation [OPP1160866]
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Chemokines are signaling proteins that are secreted to induce chemotaxis during an immunological response. However, the functions of transmembrane-type chemokine-like factor (CKLF) and the CMTM (CKLF-like MARVEL transmembrane domain containing) protein family remain to be determined. In this study, we focused on the testis-specific mouse CMTM gene cluster (Cmtm1, Cmtm2a and Cmtm2b) and generated CRISPR/Cas9-mediated mutant mice to examine their physiological functions. Although Cmtm1 mutant mice were fertile, Cmtm2a and Cmtm2b double mutant mice had defects in male fertility due to impaired sperm function. We found that co-expression of sperm membrane proteins CMTM2A and CMTM2B is required for male fertility and affects the localization of the sperm membrane protein ADAM3 in regulating sperm fertilizing ability.
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