4.5 Article

CDK2 regulates nuclear envelope protein dynamics and telomere attachment in mouse meiotic prophase

Journal

JOURNAL OF CELL SCIENCE
Volume 128, Issue 1, Pages 88-99

Publisher

COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.154922

Keywords

Meiosis; CDK2; SUN1; Telomere; Nuclear envelope

Categories

Funding

  1. Ministerio de Ciencia e Innovacion (Spain) [BFU2009-10987/BCM]
  2. Ministerio de Economia y Competitividad (Spain) [SAF2011-28842-C02-01]
  3. Priority Program SPP 1384 'Mechanisms of genome haploidization' of the German Science Foundation (DFG)
  4. Graduate School 'Organogenesis' of the University of Wurzburg (Germany)
  5. Ministerio de Economia y Competitividad (Spain)
  6. 'La Caixa' Foundation (Spain)

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In most organisms, telomeres attach to the nuclear envelope at the onset of meiosis to promote the crucial processes of pairing, recombination and synapsis during prophase I. This attachment of meiotic telomeres is mediated by the specific distribution of several nuclear envelope components that interact with the attachment plates of the synaptonemal complex. We have determined by immunofluorescence and electron microscopy that the ablation of the kinase CDK2 alters the nuclear envelope in mouse spermatocytes, and that the proteins SUN1, KASH5 (also known as CCDC155) and lamin C2 show an abnormal cap-like distribution facing the centrosome. Strikingly, some telomeres are not attached to the nuclear envelope but remain at the nuclear interior where they are associated with SUN1 and with nuclear-envelope-detached vesicles. We also demonstrate that mouse testis CDK2 phosphorylates SUN1 in vitro. We propose that during mammalian prophase I the kinase CDK2 is a key factor governing the structure of the nuclear envelope and the telomere-led chromosome movements essential for homolog pairing.

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