Journal
JOURNAL OF CELL SCIENCE
Volume 127, Issue 7, Pages 1406-1416Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.135228
Keywords
Steroid Receptor; Transcription factor; DNA binding; Single-molecule microscopy; FCS; FRAP
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Funding
- Netherlands Organization for Scientific Research (NWO-STW)
- SmartMix Program of The Netherlands Ministry of Economic Affairs
- Ministry of Education, Culture and Science
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Owing to the tremendous progress in microscopic imaging of fluorescently labeled proteins in living cells, the insight into the highly dynamic behavior of transcription factors has rapidly increased over the past decade. However, a consistent quantitative scheme of their action is still lacking. Using the androgen receptor (AR) as a model system, we combined three different fluorescence microscopy assays: single-molecule microscopy, photobleaching and correlation spectroscopy, to provide a quantitative model of the action of this transcription factor. This approach enabled us to distinguish two types of AR-DNA binding: very brief interactions, in the order of a few hundred milliseconds, and hormone-induced longer-lasting interactions, with a characteristic binding time of several seconds. In addition, freely mobile ARs were slowed down in the presence of hormone, suggesting the formation of large AR-co-regulator complexes in the nucleoplasm upon hormone activation. Our data suggest a model in which mobile hormone-induced complexes of transcription factors and co-regulators probe DNA by briefly binding at random sites, only forming relatively stable transcription initiation complexes when bound to specific recognition sequences.
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