Journal
JOURNAL OF CELL SCIENCE
Volume 127, Issue 6, Pages 1169-1178Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.130518
Keywords
microRNA; miR-149; Angiogenesis; Endothelial cell; Glypican-1; FGF2
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Funding
- National Institutes of Health [R01HL105945]
- Spanish Ministry (Programa Nacional de Movilidad de Recursos Humanos del Plan Nacional de I+D+i)
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MicroRNA-149 (miR-149) is located within the first intron of the glypican-1 (GPC1) gene. GPC1 is a low affinity receptor for fibroblast growth factor (FGF2) that enhances FGF2 binding to its receptor (FGFR1), subsequently promoting FGF2-FGFR1 activation and signaling. Using bioinformatic approaches, both GPC1 and FGFR1 were identified and subsequently validated as targets for miR-149 (both the mature strand, miR-149, and the passenger strand, miR-149*) in endothelial cells (ECs). As a consequence of their targeting activity towards GPC1 and FGFR1, both miR-149 and miR-149* regulated FGF2 signaling and FGF2-induced responses in ECs, namely proliferation, migration and cord formation. Moreover, lentiviral overexpression of miR-149 reduced in vivo tumor-induced neovascularization. Importantly, FGF2 transcriptionally stimulated the expression of miR-149 independently of its host gene, therefore assuring the steady state of FGF2-induced responses through the regulation of the GPC1-FGFR1 binary complex in ECs.
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