Journal
JOURNAL OF CELL SCIENCE
Volume 126, Issue 19, Pages 4424-4435Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.129239
Keywords
Myo10; TNTs; Dorsal filopodia; FERM domain; Intercellular transfer
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Funding
- European Union [222887]
- Agence Nationale de la Recherche (ANR) [ANR-09-BLAN-0122, ANR-09-NEUR-002-03]
- Pasteur-Weizmann Foundation
- Pasteur Foundation
- Agence Nationale de la Recherche (ANR) [ANR-09-BLAN-0122] Funding Source: Agence Nationale de la Recherche (ANR)
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Cell-to-cell communication is essential in multicellular organisms. Tunneling nanotubes (TNTs) have emerged as a new type of intercellular spreading mechanism allowing the transport of various signals, organelles and pathogens. Here, we study the role of the unconventional molecular motor myosin-X (Myo10) in the formation of functional TNTs within neuronal CAD cells. Myo10 protein expression increases the number of TNTs and the transfer of vesicles between co-cultured cells. We also show that TNT formation requires both the motor and tail domains of the protein, and identify the F2 lobe of the FERM domain within the Myo10 tail as necessary for TNT formation. Taken together, these results indicate that, in neuronal cells, TNTs can arise from a subset of Myo10-driven dorsal filopodia, independent of its binding to integrins and N-cadherins. In addition our data highlight the existence of different mechanisms for the establishment and regulation of TNTs in neuronal cells and other cell types.
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