Journal
JOURNAL OF CELL SCIENCE
Volume 125, Issue 14, Pages 3412-3420Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.100065
Keywords
ERES; COPII; ER membrane curvature
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [20001009, 22570194]
- Bioarchitect
- Extreme Photonics
- Cellular Systems Biology Projects of RIKEN
- Grants-in-Aid for Scientific Research [22570194, 24570064, 20001009] Funding Source: KAKEN
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Protein export from the endoplasmic reticulum (ER) to the Golgi apparatus occurs at specialized regions known as the ER exit sites (ERES). In Saccharomyces cerevisiae, ERES appear as numerous scattered puncta throughout the ER. We examined ERES within the peripheral ER, finding that the proteins comprising the ERES localize on high-curvature ER domains where curvature-stabilizing protein Rtn1 is present. Delta rtn1 Delta rtn2 Delta yop1 cells have fewer high-curvature ER domains, but ERES accumulate at the remaining high-curvature ER domains on the edge of expanded ER sheets. We propose that membrane curvature is a key geometric feature for the regulation of ERES localization. We also investigated a spatial relationship between ERES and Golgi cisternae. Golgi cisternae in S. cerevisiae are unstacked, dispersed, and moving in the cytoplasm with cis-cisternae positioned adjacent to ERES, whereas trans-cisternae are not. Morphological changes in the ER of Delta rtn1 Delta rtn2 Delta yop1 cells resulted in aberrant Golgi structures, including cis- and trans-markers, and there was reduced movement at ERES between expanded ER sheets and the plasma membrane.
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