Journal
JOURNAL OF CELL SCIENCE
Volume 124, Issue 3, Pages 338-347Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.078329
Keywords
CEP90; Pericentriolar satellites; Spindle pole; Centrosome
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Funding
- BioImaging Research Center at GIST
- Basic Research Program [R01-2007-000-20116-0]
- SRC Program [R11-2005-009-03005-0]
- Korean Healthcare Technology RD Project [A090558]
- Brain Korea 21 Project
- Korea Health Promotion Institute [A090558] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Ministry of Education, Science & Technology (MoST), Republic of Korea [gist-12] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2011-0006425] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Pericentriolar satellites are electron-dense granules that are concentrated around the centrosome. They are involved in the recruitment of centrosomal proteins and microtubule organization in interphase cells, but their mitotic functions are largely unknown. In this study, we characterize CEP90 as a component of pericentriolar satellites. CEP90 is present both in the centrosome and in the cytoplasm, but is transiently concentrated at the centrosome once cells enter mitosis. Depletion of CEP90 caused mitotic arrest with misaligned chromosomes. Spindle pole fragmentation was the most characteristic phenotype in CEP90-depleted cells. Spindle poles were fragmented as soon as the spindles attached, suggesting that the mechanical forces of spindle microtubules physically stress the structure of CEP90-depleted spindle poles. Based on these results, we propose that CEP90 is crucial for maintaining the integrity of spindle poles during mitosis.
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