Journal
JOURNAL OF CELL SCIENCE
Volume 124, Issue 8, Pages 1328-1338Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.079194
Keywords
Meiotic recombination; Spo11-accessory proteins; Recombination-initiation complex; Fission yeast
Categories
Funding
- National Institutes of Health of the United States of America [GM032194]
- Spanish Ministry of Science and Innovation [CSD2007-00015, FEDER-BFU2008-01808, FEDER-BFU2007-66366, FEDER-BFU2010-14954]
- Junta de Castilla y Leon [GR265, SA038A07]
- CSIC [PII-200820I125]
Ask authors/readers for more resources
A physical connection between each pair of homologous chromosomes is crucial for reductional chromosome segregation during the first meiotic division and therefore for successful meiosis. Connection is provided by recombination (crossing over) initiated by programmed DNA double-strand breaks (DSBs). Although the topoisomerase-like protein Spo11 makes DSBs and is evolutionarily conserved, how Spo11 (Rec12 in fission yeast) is regulated to form DSBs at the proper time and place is poorly understood. Several additional (accessory) proteins for DSB formation have been inferred in different species from yeast to mice. Here, we show that Rec24 is a bona fide accessory protein in Schizosaccharomyces pombe. Rec24 is required genome-wide for crossing-over and is recruited to meiotic chromosomes during prophase in a Rec12-independent manner forming foci on linear elements (LinEs), structurally related to the synaptonemal complex of other eukaryotes. Stabilization of Rec24 on LinEs depends on another accessory protein, Rec7, with which Rec24 forms complexes in vivo. We propose that Rec24 marks LinE-associated recombination sites, that stabilization of its binding by Rec7 facilitates the loading or activation of Rec12, and that only stabilized complexes containing Rec24 and Rec7 promote DSB formation. Based on the recent report of Rec24 and Rec7 conservation, interaction between Rec24 and Rec7 might be widely conserved in DSB formation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available