Journal
JOURNAL OF CELL SCIENCE
Volume 123, Issue 9, Pages 1522-1530Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.055970
Keywords
Tail-anchored protein; Asna1/TRC40; RAMP4; Sec61 beta; Cytochrome b5; Endoplasmic reticulum
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Funding
- Deutsche Forschungsgemeinschaft [SFB638]
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Tail-anchored (TA) proteins insert post-translationally into the membrane of the endoplasmic reticulum (ER) and span the membrane by their C-terminal transmembrane domain. We have reconstituted membrane insertion of TA proteins from recombinant Asna1/TA protein complexes and ER-derived membranes. Our data show that Asna1 can mediate membrane insertion of RAMP4 and Sec61. without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the ER membrane. By contrast, membrane insertion of cytochrome b5 can proceed independently of Asna1 and nucleotides.
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