Journal
JOURNAL OF CELL SCIENCE
Volume 123, Issue 21, Pages 3734-3744Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.070466
Keywords
Non-coding RNAs; Nuclear domains; Nuclear matrix; Triplex repeats; Midbody
Categories
Funding
- [UIUC-ICR-MCB]
- [NSF0843604]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [0843604] Funding Source: National Science Foundation
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In higher eukaryotic cells, long non-protein-coding RNAs (lncRNAs) have been implicated in a wide array of cellular functions. Cell-or tissue-specific expression of lncRNA genes encoded in the mammalian genome is thought to contribute to the complex gene networks needed to regulate cellular function. Here, we have identified a novel species of polypurine triplet repeat-rich lncRNAs, designated as GAA repeat-containing RNAs (GRC-RNAs), that localize to numerous punctate foci in the mammalian interphase nuclei. GRC-RNAs consist of a heterogeneous population of RNAs, ranging in size from similar to 1.5 kb to similar to 4 kb and localize to subnuclear domains, several of which associate with GAA. TTC-repeat-containing genomic regions. GRC-RNAs are components of the nuclear matrix and interact with various nuclear matrix-associated proteins. In mitotic cells, GRC-RNAs form distinct cytoplasmic foci and, in telophase and G1 cells, localize to the midbody, a structure involved in accurate cell division. Differentiation of tissue culture cells leads to a decrease in the number of GRC-RNA nuclear foci, albeit with an increase in size as compared with proliferating cells. Conversely, the number of GRC-RNA foci increases during cellular transformation. We propose that nuclear GRC-RNAs represent a novel family of mammalian lncRNAs that might play crucial roles in the cell nucleus.
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