Journal
JOURNAL OF CELL SCIENCE
Volume 122, Issue 10, Pages 1551-1562Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.046284
Keywords
Nuclear organization; Nuclear compartments; Nucleoskeleton; Nuclear lamins; Nucleoli; Fibrillarin; Nucleophosmin/B23
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Funding
- BBSRC [D00327X]
- BBSRC [BB/D00327X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D00327X/1] Funding Source: researchfish
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The dynamic ability of genomes to interact with discrete nuclear compartments appears to be essential for chromatin function. However, the extent to which structural nuclear proteins contribute to this level of organization is largely unresolved. To test the links between structure and function, we evaluated how nuclear lamins contribute to the organization of a major functional compartment, the nucleolus. HeLa cells with compromised expression of the genes encoding lamins were analyzed using high-resolution imaging and pull-down assays. When lamin B1 expression was depleted, inhibition of RNA synthesis correlated with complex structural changes within the nucleolar active centers until, eventually, the nucleoli were dispersed completely. With normal lamin expression, the nucleoli were highly plastic, with dramatic and freely reversible structural changes correlating with the demand for ribosome biogenesis. Preservation of the nucleolar compartment throughout these structural transitions is shown to be linked to lamin B1 expression, with the lamin B1 protein interacting with the major nucleolar protein nucleophosmin/B23.
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