Journal
JOURNAL OF CELL SCIENCE
Volume 122, Issue 9, Pages 1306-1314Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.045096
Keywords
Cilia; Mass spectroscopy; Immunofluorescence microscopy; Phylogeny
Categories
Ask authors/readers for more resources
The movements of cilia and flagella are driven by multiple species of dynein heavy chains (DHCs), which constitute inner- and outer-dynein arms. In Chlamydomonas, 11 DHC proteins have been identified in the axoneme, but 14 genes encoding axonemal DHCs are present in the genome. Here, we assigned each previously unassigned DHC gene to a particular DHC protein and found that DHC3, DHC4 and DHC11 encode novel, relatively low abundance DHCs. Immunofluorescence microcopy revealed that DHC11 is localized exclusively to the proximal similar to 2 mu m region of the similar to 12 mu m long flagellum. Analyses of growing flagella suggested that DHC3 and DHC4 are also localized to the proximal region. By contrast, the DHC of a previously identified inner-arm dynein, dynein b, displayed an inverse distribution pattern. Thus, the proximal portion of the flagellar axoneme apparently differs in dynein composition from the remaining portion; this difference might be relevant to the special function performed by the flagellar base.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available