Journal
JOURNAL OF CELL SCIENCE
Volume 122, Issue 22, Pages 4168-4176Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.054122
Keywords
Chromosome cohesion; Centrosome; Mitosis; Gsg2 Spindle assembly; Kinesin-5
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Funding
- NIGMS NIH HHS [R01 GM074210, R01 GM074210-04] Funding Source: Medline
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Cohesins and their regulators are vital for normal chromosome cohesion and segregation. A number of cohesion proteins have also been localized to centrosomes and proposed to function there. We show that RNAi-mediated depletion of factors required for cohesion, including haspin, Sgo1 and Scc1, leads to the generation of multiple acentriolar centrosome-like foci and disruption of spindle structure in mitosis. Live-cell imaging reveals that, in haspin-depleted cells, these effects occur only as defects in chromosome cohesion become manifest, and they require ongoing microtubule dynamics and kinesin-5 (also known as Eg5) activity. Inhibition of topoisomerase Pi in mitosis, which prevents decatenation and separation of chromatids, circumvents the loss of cohesion and restores integrity of the spindle poles. Although these results do not rule out roles for cohesin proteins at centrosomes, they suggest that when cohesion is compromised, spindle-pole integrity can be disrupted as an indirect consequence of the failure to properly integrate chromosome-and centrosome-initiated pathways for spindle formation.
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