Journal
JOURNAL OF CELL SCIENCE
Volume 122, Issue 16, Pages 2895-2905Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.051607
Keywords
KASH protein; Outer nuclear membrane; Nuclear envelope; Cell cycle; SUN protein; C. elegans
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Funding
- Canadian Foundation for Innovation (CFI)
- Canadian Institute for Health Research (CIHR)
- Canadian Cancer Society
- Heart and Stroke Foundation of Canada
- Novartis
- National Institutes of Health [R01GM073874]
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Klarsicht, ANC-1 and Syne homology (KASH) proteins localize to the outer nuclear membrane where they connect the nucleus to the cytoskeleton. KASH proteins interact with Sad1-UNC-84 (SUN) proteins to transfer forces across the nuclear envelope to position nuclei or move chromosomes. A new KASH protein, KDP-1, was identified in a membrane yeast two-hybrid screen of a Caenorhabditis elegans library using the SUN protein UNC-84 as bait. KDP-1 also interacted with SUN-1. KDP-1 was enriched at the nuclear envelope in a variety of tissues and required SUN-1 for nuclear envelope localization in the germline. Genetic analyses showed that kdp-1 was essential for embryonic viability, larval growth and germline development. kdp-1(RNAi) delayed the entry into mitosis in embryos, led to a small mitotic zone in the germline, and caused an endomitotic phenotype. Aspects of these phenotypes were similar to those seen in sun-1(RNAi), suggesting that KDP-1 functions with SUN-1 in the germline and early embryo. The data suggest that KDP-1 is a novel KASH protein that functions to ensure the timely progression of the cell cycle between the end of S phase and the entry into mitosis.
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