Journal
JOURNAL OF CELL SCIENCE
Volume 122, Issue 10, Pages 1540-1550Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.042457
Keywords
ER; Golgi; COPII vesicles; Trafficking; Yip1 proteins
Categories
Funding
- National Institutes of Health
Ask authors/readers for more resources
Yip1p belongs to a conserved family of membrane-spanning proteins that are involved in intracellular trafficking. Studies have shown that Yip1p forms a heteromeric integral membrane complex, is required for biogenesis of ER-derived COPII vesicles, and can interact with Rab GTPases. However, the role of the Yip1 complex in vesicle budding is not well understood. To gain further insight, we isolated multicopy suppressors of the thermosensitive yip1-2 allele. This screen identified GOT1, FYV8 and TSC3 as novel high-copy suppressors. The strongest suppressor, GOT1, also displayed moderate suppressor activity toward temperature-sensitive mutations in the SEC23 and SEC31 genes, which encode subunits of the COPII coat. Further characterization of Got1p revealed that this protein was efficiently packaged into COPII vesicles and cycled rapidly between the ER and Golgi compartments. Based on the findings we propose that Got1p has an unexpected role in vesicle formation from the ER by influencing membrane properties.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available