Journal
JOURNAL OF CELL SCIENCE
Volume 121, Issue 17, Pages 2913-2920Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.023911
Keywords
cyclase-associated protein; srv2; ASP-56; reactive oxygen species; cytochrome c; staurosporine; etoposide
Categories
Funding
- American Heart Association [0630394N]
- Hong Kong Research Grant Council [HKUST6242/04M]
- NIH [GM48241]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM048241] Funding Source: NIH RePORTER
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Mitochondria play a central role in regulating apoptosis by releasing proapoptotic contents such as cytochrome c, and generating reactive oxygen species (ROS). Early in apoptosis, proteins translocate to mitochondria to promote the release of their contents. Here, we show that the actin- and cofilin-interacting protein CAP1 has a role in apoptosis. When we induced apoptosis, CAP1 rapidly translocated to the mitochondria independently of caspase activation. Translocation was proapoptotic because CAP1-knockdown cells were resistant to apoptosis inducers. Overexpression of wild-type CAP1 did not stimulate apoptosis on its own, but stimulated cofilin-induced apoptosis. Apoptosis induction required a mitochondrial-targeting domain, localized in the N-terminus and also the actin- binding domain in the C-terminus. Taken together, these studies suggest that CAP1 provides a direct link from the actin cytoskeleton to the mitochondria by functioning as an actin shuttle.
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