Journal
JOURNAL OF CELL SCIENCE
Volume 121, Issue 4, Pages 428-436Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.015826
Keywords
intraflagellar transport; cilia/flagella; ciliary assembly; WD domain protein; gene targeting
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Funding
- NIGMS NIH HHS [GM 26973] Funding Source: Medline
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Intraflagellar transport (IFT) moves multiple protein particles composed of two biochemically distinct complexes, IFT-A and IFT-B, bi-directionally within cilia and is essential for cilia assembly and maintenance. We identified an ORF from the Tetrahymena macronuclear genome sequence, encoding IFT122A, an ortholog of an IFT-A complex protein. Tetrahymena IFT122A is induced during cilia regeneration, and epitope-tagged Ift122Ap could be detected in isolated cilia. IFT122A knockout cells still assembled cilia, albeit with lower efficiency, and could regenerate amputated cilia. Ift172p and Ift88p, two IFT-B complex proteins that localized mainly to basal bodies and along the cilia in wild-type cells, became preferentially enriched at the ciliary tips in IFT122A knockout cells. Our results indicate that Tetrahymena IFT122A is not required for anterograde transport-dependent ciliary assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body.
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