Journal
JOURNAL OF CELL BIOLOGY
Volume 204, Issue 5, Pages 669-682Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201308029
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Funding
- National Institutes of Health [PO1 DK032094, RO1HL062352, R01EB007049]
- National Science Foundation [1200834]
- Human Frontier Science Program
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1200834] Funding Source: National Science Foundation
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Cell migration through solid tissue often involves large contortions of the nucleus, but biological significance is largely unclear. The nucleoskeletal protein lamin-A varies both within and between cell types and was shown here to contribute to cell sorting and survival in migration through constraining micropores. Lamin-A proved rate-limiting in 3D migration of diverse human cells that ranged from glioma and adenocarcinoma lines to primary mesenchymal stem cells (MSCs). Stoichiometry of A- to B-type lamins established an activation barrier, with high lamin-A:B producing extruded nuclear shapes after migration. Because the juxtaposed A and B polymer assemblies respectively conferred viscous and elastic stiffness to the nucleus, subpopulations with different A:B levels sorted in 3D migration. However, net migration was also biphasic in lamin-A, as wild-type lamin-A levels protected against stress-induced death, whereas deep knockdown caused broad defects in stress resistance. In vivo xenografts proved consistent with A:B-based cell sorting, and intermediate A:B-enhanced tumor growth. Lamins thus impede 3D migration but also promote survival against migration-induced stresses.
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