Journal
JOURNAL OF CELL BIOLOGY
Volume 205, Issue 3, Pages 313-324Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201312024
Keywords
-
Categories
Funding
- Pew Scholars Program in the Biomedical Sciences
- National Institutes of Health National Institute of General Medical Sciences [GM-100122]
- American Cancer Society [124521-PF-13-109-01-CCG]
Ask authors/readers for more resources
During cell division, a mitotic spindle is built by the cell and acts to align and stretch duplicated sister chromosomes before their ultimate segregation into daughter cells. Stretching of the pericentromeric chromatin during metaphase is thought to generate a tension-based signal that promotes proper chromosome segregation. However, it is not known whether the mitotic spindle actively maintains a set point tension magnitude for properly attached sister chromosomes to facilitate robust mechanochemical checkpoint signaling. By imaging and tracking the thermal movements of pericentromeric fluorescent markers in Saccharomyces cerevisiae, we measured pericentromere stiffness and then used the stiffness measurements to quantitatively evaluate the tension generated by pericentromere stretch during metaphase in wild-type cells and in mutants with disrupted chromosome structure. We found that pericentromere tension in yeast is substantial (4-6 pN) and is tightly self-regulated by the mitotic spindle: through adjustments in spindle structure, the cell maintains wild-type tension magnitudes even when pericentromere stiffness is disrupted.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available