Journal
JOURNAL OF CELL BIOLOGY
Volume 206, Issue 7, Pages 833-842Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201406109
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Funding
- Medical Research Council Senior Non-Clinical Research fellowship [MR/K006703/1]
- Cancer Research UK Career Development fellowship [C24085/A8296]
- Cancer Research UK program [C20079/A15940]
- EP Abraham Research Fund studentship
- Wellcome Trust Strategic Award [091911]
- Medical Research Council [MR/K006703/1] Funding Source: researchfish
- MRC [MR/K006703/1] Funding Source: UKRI
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The spindle assembly checkpoint (SAC) monitors correct attachment of chromosomes to microtubules, an important safeguard mechanism ensuring faithful chromosome segregation in eukaryotic cells. How the SAC signal is turned off once all the chromosomes have successfully attached to the spindle remains an unresolved question. Mps1 phosphorylation of Knl1 results in recruitment of the SAC proteins Bub1, Bub3, and BubR1 to the kinetochore and production of the wait-anaphase signal. SAC silencing is therefore expected to involve a phosphatase opposing Mps1. Here we demonstrate in vivo and in vitro that BubR1-associated PP2A-B56 is a key phosphatase for the removal of the Mps1-mediated Knl1 phosphorylations necessary for Bub1/BubR1 recruitment in mammalian cells. SAC silencing is thus promoted by a negative feedback loop involving the Mps1-dependent recruitment of a phosphatase opposing Mps1. Our findings extend the previously reported role for BubR1-associated PP2A-B56 in opposing Aurora B and suggest that BubR1-bound PP2A-B56 integrates kinetochore surveillance and silencing of the SAC.
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