Journal
JOURNAL OF CELL BIOLOGY
Volume 201, Issue 4, Pages 559-575Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201209107
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Funding
- NIH National Center of Research Resources
- Swiss National Science Foundation
- NCCR Chemical Biology
- University of Geneva
- Ernst & Lucie Schmidheiny foundation
- Ernest Boninchi foundation
- Novartis foundation
- ETH Zurich
- JSPS KAKENHI [19671003]
- Funding Program for Next Generation World-Leading Researchers [LS006]
- Deutsche Forschungsgemeinschaft [ME 1626/3-1]
- Grants-in-Aid for Scientific Research [19671003] Funding Source: KAKEN
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Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.
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