Journal
JOURNAL OF CELL BIOLOGY
Volume 199, Issue 3, Pages 497-512Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201201091
Keywords
-
Categories
Funding
- National Institutes of Health [R01AI44902, R01HL65333]
- American Heart Association
- National Natural Science Foundation of China [31070827]
- Chinese Academy of Sciences
Ask authors/readers for more resources
As adhesion molecules, integrins connect a cell to its environment and transduce signals across the membrane. Their different functional states correspond to distinct conformations. Using a biomembrane force probe, we observed real-time reversible switches between bent and extended conformations of a single integrin, alpha(L)beta(2), on the surface of a living cell by measuring its nanometer-scale headpiece displacements, bending and unbending frequencies, and molecular stiffness changes. We determined the stabilities of these conformations, their dynamic equilibrium, speeds and rates of conformational changes, and the impact of divalent cations and tensile forces. We quantified how initial and subsequent conformations of alpha(L)beta(2) regulate the force-dependent kinetics of dissociation from intercellular adhesion molecule 1. Our findings provide new insights into how integrins function as nanomachines to precisely control cell adhesion and signaling.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available