Journal
JOURNAL OF CELL BIOLOGY
Volume 195, Issue 7, Pages 1141-1157Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201108159
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Funding
- University of Buenos Aires, Argentina [UBACyT X834, UBACyT X311]
- Consejo Nacional de Investigaciones Cientificas y Tecnologicas (CONICET) [PIP 6173]
- Agencia Nacional de Promocion Cientifica y Tecnologica, (ANPCyT), Argentina [PICT 38006, PICT 1965]
- National Institutes of Health [1R03TW006037-01A1]
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Mammalian Smaug1/Samd4A is a translational repressor. Here we show that Smaug1 forms mRNA-silencing foci located at postsynapses of hippocampal neurons. These structures, which we have named S-foci, are distinct from P-bodies, stress granules, or other neuronal RNA granules hitherto described, and are the first described mRNA-silencing foci specific to neurons. RNA binding was not required for aggregation, which indicates that S-foci formation is not a consequence of mRNA silencing. N-methyl-d-aspartic acid (NMDA) receptor stimulation provoked a rapid and reversible disassembly of S-foci, transiently releasing transcripts (the CaMKII. mRNA among others) to allow their translation. Simultaneously, NMDA triggered global translational silencing, which suggests the specific acti-vation of Smaug1-repressed transcripts. Smaug1 is expressed during synaptogenesis, and Smaug1 knockdown affected the number and size of synapses, and also provoked an impaired response to repetitive depolarizing stimuli, as indicated by a reduced induction of Arc/Arg3.1. Our results suggest that S-foci control local translation, specifically responding to NMDA receptor stimulation and affecting synaptic plasticity.
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