Journal
JOURNAL OF CELL BIOLOGY
Volume 189, Issue 4, Pages 713-723Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200910142
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Funding
- Murdock Charitable Trust
- Washington Research Foundation
- National Sciences and Engineering Research Council of Canada
- National Institutes of Health [T32 GM008268]
- National Science Foundation [DGE-0504573]
- Searle Scholar award [06-L-111]
- Packard Fellowship for Science and Engineering [2006-30521]
- National Institute of General Medical Sciences [R01GM40506, R01GM79373]
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The coupling of kinetochores to dynamic spindle microtubules is crucial for chromosome positioning and segregation, error correction, and cell cycle progression. How these fundamental attachments are made and persist under tensile forces from the spindle remain important questions. As microtubule-binding elements, the budding yeast Ndc80 and Dam1 kinetochore complexes are essential and not redundant, but their distinct contributions are unknown. In this study, we show that the Dam1 complex is a processivity factor for the Ndc80 complex, enhancing the ability of the Ndc80 complex to form load-bearing attachments to and track with dynamic microtubule tips in vitro. Moreover, the interaction between the Ndc80 and Dam1 complexes is abolished when the Dam1 complex is phosphorylated by the yeast aurora B kinase Ipl1. This provides evidence for a mechanism by which aurora B resets aberrant kinetochore-microtubule attachments. We propose that the action of the Dam1 complex as a processivity factor in kinetochore-microtubule attachment is regulated by conserved signals for error correction.
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