Journal
JOURNAL OF CELL BIOLOGY
Volume 185, Issue 7, Pages 1285-1298Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200902147
Keywords
-
Categories
Funding
- VUMC Training Program in Developmental Biology, American Heart Association [HD07502]
- National Institutes of Health [DK-075555, CA-46413, CA-126218]
- Special Program of Research Excellence [NCI P50 95103]
- Mouse Models of Human Cancers Consortium [U01 084239]
- Vanderbilt Digestive Diseases Research Core [P30 DK-058404]
Ask authors/readers for more resources
For decades, enterocyte brush border microvilli have been viewed as passive cytoskeletal scaffolds that serve to increase apical membrane surface area. However, recent studies revealed that in the in vitro context of isolated brush borders, myosin-1a (myo1a) powers the sliding of microvillar membrane along core actin bundles. This activity also leads to the shedding of small vesicles from microvillar tips, suggesting that microvilli may function as vesicle-generating organelles in vivo. In this study, we present data in support of this hypothesis, showing that enterocyte microvilli release unilamellar vesicles into the intestinal lumen; these vesicles retain the right side out orientation of microvillar membrane, contain catalytically active brush border enzymes, and are specifically enriched in intestinal alkaline phosphatase. Moreover, myo1a knockout mice demonstrate striking perturbations in vesicle production, clearly implicating this motor in the in vivo regulation of this novel activity. In combination, these data show that microvilli function as vesicle-generating organelles, which enable enterocytes to deploy catalytic activities into the intestinal lumen.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available