Journal
JOURNAL OF CELL BIOLOGY
Volume 186, Issue 2, Pages 283-295Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200905083
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Funding
- National Institutes of Health [GM51293]
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A system distinct from the central pair-radial spoke complex was proposed to control outer arm dynein function in response to alterations in the mechanical state of the flagellum. In this study, we examine the role of a Chlamydomonas reinhardtii outer arm dynein light chain that associates with the motor domain of the. heavy chain (HC). We demonstrate that expression of mutant forms of LC1 yield dominant-negative effects on swimming velocity, as the flagella continually beat out of phase and stall near or at the power/recovery stroke switchpoint. Furthermore, we observed that LC1 interacts directly with tubulin in a nucleotide-independent manner and tethers this motor unit to the A-tubule of the outer doublet microtubules within the axoneme. Therefore, this dynein HC is attached to the same microtubule by two sites: via both the N-terminal region and the motor domain. We propose that this. HC-LC1-microtubule ternary complex functions as a conformational switch to control outer arm activity.
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