Journal
JOURNAL OF CELL BIOLOGY
Volume 185, Issue 2, Pages 225-233Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200809144
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Funding
- Howard Hughes Medical Institute
- National Institutes of Health Molecular Therapy Core Center [P30 DK065988, P01HL034322]
- National Institutes of Health [R01 DK073191]
- Cystic Fibrosis Foundation Research Development Program
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Chibby (Cby) is a conserved component of the Wnt-beta-catenin pathway. Cby physically interacts with.-catenin to repress its activation of transcription. To elucidate the function of Cby in vertebrates, we generated Cby(-/-) mice and found that after 2-3 d of weight loss, the majority of mice die before or around weaning. All Cby(-/-) mice develop rhinitis and sinusitis. When challenged with Pseudomonas aeruginosa isolates, Cby(-/-) mice are unable to clear the bacteria from the nasal cavity. Notably, Cby(-/-) mice exhibit a complete absence of mucociliary transport caused by a marked paucity of motile cilia in the nasal epithelium. Moreover, ultrastructural experiments reveal impaired basal body docking to the apical surface of multiciliated cells. In support of these phenotypes, endogenous Cby protein is localized at the base of cilia. As the phenotypes of Cby(-/-) mice bear striking similarities to primary ciliary dyskinesia, Cby(-/-) mice may prove to be a useful model for this condition.
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