Journal
JOURNAL OF CELL BIOLOGY
Volume 185, Issue 6, Pages 995-1012Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200903125
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Funding
- Swarthmore College
- Howard Hughes Medical Institute
- Swarthmore College [52002999, 52002654, 52005202]
- National Institutes of Health [R15 GM065883-01, GM59216, GM56846, GM31006]
- American Heart Association [0715300U]
- American Cancer Society [RSG-02-039-01-CSM]
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Cytokinesis requires coordination of actomyosin ring (AMR) contraction with rearrangements of the plasma membrane and extracellular matrix. In Saccharomyces cerevisiae, new membrane, the chitin synthase Chs2 (which forms the primary septum [PS]), and the protein Inn1 are all delivered to the division site upon mitotic exit even when the AMR is absent. Inn1 is essential for PS formation but not for Chs2 localization. The Inn1 C-terminal region is necessary for localization, and distinct PXXP motifs in this region mediate functionally important interactions with SH3 domains in the cytokinesis proteins Hof1 (an F-BAR protein) and Cyk3 (whose overexpression can restore PS formation in inn1. cells). The Inn1 N terminus resembles C2 domains but does not appear to bind phospholipids; nonetheless, when over-expressed or fused to Hof1, it can provide Inn1 function even in the absence of the AMR. Thus, Inn1 and Cyk3 appear to cooperate in activating Chs2 for PS formation, which allows coordination of AMR contraction with ingression of the cleavage furrow.
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