Journal
JOURNAL OF CATARACT AND REFRACTIVE SURGERY
Volume 36, Issue 4, Pages 659-664Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jcrs.2009.11.003
Keywords
-
Categories
Funding
- Department of Surgery, School of Medicine, Universidad Nacional de Colombia, Bogota
Ask authors/readers for more resources
PURPOSE: To evaluate the effect of genipin, a natural crosslinker, on porcine corneas SETTING: Department of Ophthalmology, Universidad Nacional de Colombia, Bogota, Colombia METHODS: Corneal strips (12.0 mm x 2 3 mm) were harvested from porcine eyes and treated by incubation with genipin at concentrations of 1.00%, 0.25%, and 010%. Parallel corneal strips from the same eye were used as untreated controls After treatment at 20 degrees C for 40 minutes, tensile strain measurements were performed in a biomaterial tester. Porcine button corneas were treated with genipin 0 25% for 15 minutes and then digested by bacterial collagenase. Treated and untreated corneas were evaluated by light microscopy. RESULTS: Young modulus and stiffness in treated corneas increased in a concentration-dependent manner Genipin increased resistance to corneal collagenase 5-fold in comparison with the controls. A decrease in the interlamellar space in treated corneas was also observed. CONCLUSIONS: Corneal collagen crosslinking induced with genipin produced a significant increase in biomechanical strength and resistance to bacterial collagenase This crosslinker could be useful in treating corneal ectasia and corneal infectious and noninfectious diseases involving corneal melting.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available