Journal
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
Volume 107, Issue 4, Pages 921-933Publisher
SPRINGER
DOI: 10.1007/s10482-015-0384-8
Keywords
Post-Light (TM) ion semiconductor sequencing; Microbial functional prediction; Microbial phylogeny; Africanized honeybee; Apis mellifera; 16S rRNA
Categories
Funding
- National Council for Scientific and Technological Development (CNPq-Brazil)
- Coordination for the Improvement of Higher Education Personnel (CAPES-Brazil)
- Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS-Brazil)
- Programa de Apoio aos Polos Tecnologicos
- CNPq
- [Process 474-2500/13-9]
Ask authors/readers for more resources
The microbiota and the functional genes actively involved in the process of breakdown and utilization of pollen grains in beebread and bee guts are not yet understood. The aim of this work was to assess the diversity and community structure of bacteria and archaea in Africanized honeybee guts and beebread as well as to predict the genes involved in the microbial bioprocessing of pollen using state of the art 'post-light' based sequencing technology. A total of 11 bacterial phyla were found within bee guts and 10 bacterial phyla were found within beebread. Although the phylum level comparison shows most phyla in common, a deeper phylogenetic analysis showed greater variation of taxonomic composition. The families Enterobacteriaceae, Ricketsiaceae, Spiroplasmataceae and Bacillaceae, were the main groups responsible for the specificity of the bee gut while the main families responsible for the specificity of the beebread were Neisseriaceae, Flavobacteriaceae, Acetobacteraceae and Lactobacillaceae. In terms of microbial community structure, the analysis showed that the communities from the two environments were quite different from each other with only 7 % of species-level taxa shared between bee gut and beebread. The results indicated the presence of a highly specialized and well-adapted microbiota within each bee gut and beebread. The beebread community included a greater relative abundance of genes related to amino acid, carbohydrate, and lipid metabolism, suggesting that pollen biodegradation predominantly occurs in the beebread. These results suggests a complex and important relationship between honeybee nutrition and their microbial communities.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available