Journal
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
Volume 135, Issue 10, Pages 1351-1358Publisher
SPRINGER
DOI: 10.1007/s00432-009-0576-6
Keywords
Urothelial carcinoma of the urinary bladder; Urinary tenascin-C; Splicing domains; ELISA; Invasion; Tumour surveillance
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Funding
- Thuringian Ministry of Science, Research and Art
- University Jena [B307-04004]
- European Community [FP7/2007-2013, Health-F2-2008-201342]
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Surveillance of urothelial carcinoma of the urinary bladder (UBC) patients with respect to tumour recurrence and invasiveness is crucial for therapy and prognosis. Therefore, evaluation of non-invasive methods to monitor tumour progression is of high clinical interest. The study was aimed at investigating urinary concentrations of tenascin-C splicing domains for their value as tumour surveillance markers. Urinary concentration of B and C domain containing tenascin-C (Tn-C) was analysed by ELISA technology in 104 UBC patients, 11 patients with cystitis and 15 healthy donors as control. The investigation was supplemented by Tn-C immunohistochemistry and Western blotting. A statistically significant increase in urinary concentrations of both Tn-C B and C domain with tumour progression could be evidenced. A concordant tumour-associated enhanced protein deposition in the carcinoma stroma could be demonstrated by immunohistochemistry in invasive UBC. Western blotting reveals proteolytic fragmentation of urinary Tn-C. In summary, detection of Tn-C splicing domains in urine is suggested as a marker for the surveillance of UBC recurrence and invasiveness.
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