Journal
NEUROBIOLOGY OF AGING
Volume 36, Issue 12, Pages 3321-3333Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.neurobiolaging.2015.08.024
Keywords
Parkinson's disease; Autopsied human midbrains; Stereology; Morphometry; Densitometry; GFAP; CNPase; Iba1; S100 beta
Categories
Funding
- Council for Scientific and Industrial Research, Government of India [CSIR] [27(0884)/09]
- ICMR [5/4-5/8/Neuro/2009-NCD-I]
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Age being a risk factor for Parkinson's disease, assessment of age-related changes in the human substantia nigra may elucidate its pathogenesis. Increase in Marinesco bodies, alpha-synuclein, free radicals and so forth in the aging nigral neurons are clear indicators of neurodegeneration. Here, we report the glial responses in aging human nigra. The glial numbers were determined on Nissl-stained sections. The expression of glial fibrillary acidic protein, S100 beta, 2', 3'-cyclic nucleotide 3' phosphodiesterase, and Iba1 was assessed on cryosections of autopsied midbrains by immunohistochemistry and densitometry. The glial counts showed a biphasic increase, of which, the first prominent phase from fetal age to birth could be physiological gliogenesis whereas the second one after middle age may reflect mild age-related gliosis. Astrocytic morphology was altered, but glial fibrillary acidic protein expression increased only mildly. Presence of type-4 microglia suggests possibility of neuroinflammation. Mild reduction in 2', 3'-cyclic nucleotide 3' phosphodiesterase-labeled area denotes subtle demyelination. Stable age-related S100 beta expression indicates absence of calcium overload. Against the expected prominent gliosis, subtle age-related morphological alterations in human nigral glia attribute them a participatory role in aging. (C) 2015 Elsevier Inc. All rights reserved.
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