Journal
JOURNAL OF BONE AND MINERAL RESEARCH
Volume 28, Issue 3, Pages 660-671Publisher
WILEY-BLACKWELL
DOI: 10.1002/jbmr.1778
Keywords
H1 CALPONIN; BONE REMODELING; CYTOSKELETON; OSTEOBLAST; OSTEOCLAST
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Funding
- National Natural Science Foundation of China [81030036, 81270012, 30901527]
- Special Funds for Major State Basic Research Program of China (973 program) [2011CB964701, 2012CB518106]
- Committee of Science and Technology of Chongqing (CSTC [2011jjA1468, SKLZZ201017]
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H1 calponin (CNN1) is known as a smooth muscle-specific, actin-binding protein which regulates smooth muscle contractive activity. Although previous studies have shown that CNN1 has effect on bone, the mechanism is not well defined. To investigate the role of CNN1 in maintaining bone homeostasis, we generated transgenic mice overexpressing Cnn1 under the control of the osteoblast-specific 3.6-kb Col1a1 promoter. Col1a1-Cnn1 transgenic mice showed delayed bone formation at embryonic stage and decreased bone mass at adult stage. Morphology analyses showed reduced trabecular number, thickness and defects in bone formation. The proliferation and migration of osteoblasts were decreased in Col1a1-Cnn1 mice due to alterations in cytoskeleton. The early osteoblast differentiation of Col1a1-Cnn1 mice was increased, but the late stage differentiation and mineralization of osteoblasts derived from Col1a1-Cnn1 mice were significantly decreased. In addition to impaired bone formation, the decreased bone mass was also associated with enhanced osteoclastogenesis. Tartrate-resistant acid phosphatase (TRAP) staining revealed increased osteoclast numbers in tibias of 2-month-old Col1a1-Cnn1 mice, and increased numbers of osteoclasts co-cultured with Col1a1-Cnn1 osteoblasts. The ratio of RANKL to OPG was significantly increased in Col1a1-Cnn1 osteoblasts. These findings reveal a novel function of CNN1 in maintaining bone homeostasis by coupling bone formation to bone resorption. (c) 2013 American Society for Bone and Mineral Research.
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