4.6 Article

Mutations in the insulin-like factor 3 receptor are associated with osteoporosis

Journal

JOURNAL OF BONE AND MINERAL RESEARCH
Volume 23, Issue 5, Pages 683-693

Publisher

AMER SOC BONE & MINERAL RES
DOI: 10.1359/JBMR.080204

Keywords

osteoporosis; insulin-like factor 3; relaxin family peptide; hypogonadism; leucine-rich repeat-containing G protein-coupled receptor 8

Funding

  1. NIAMS NIH HHS [AR051459, R03 AR051459] Funding Source: Medline
  2. NICHD NIH HHS [R01 HD37067, U54 HD28934, U54 HD028934, R01 HD037067] Funding Source: Medline
  3. Telethon [TCP00089] Funding Source: Medline

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Introduction: Insulin-like factor 3 (INSL3) is produced primarily by testicular Leydig cells. It acts by binding to its specific G protein-coupled receptor RXFP2 (relaxin family peptide 2) and is involved in testicular descent during fetal development. The physiological role of INSL3 in adults is not known, although substantial INSL3 circulating levels are present. The aim of this study was to verify whether reduced INSL3 activity could cause or contribute to some signs of hypogonadism, such as reduced BMD, currently attributed to testosterone deficiency. Materials and Methods: Extensive clinical, biochemical, and hormonal study, including bone densitometry by DXA, was performed on 25 young men (age, 27-41 yr) with the well-characterized T222P mutation in the RXFP2 gene. Expression analysis of INSL3 and RXFP2 on human bone biopsy and human and mouse ostcoblast cell cultures was performed by RT-PCR, quantitative RT-PCR, and immunohistochemistry. Realtime cAMP imaging analysis and proliferation assay under the stimulus of INSL3 was performed on these cells. Lumbar spine and femoral bone of Rxfp2-deficient mice were studied by static and dynamic histomorphometry and mu CT, respectively. Results: Sixteen of 25 (64%) young men with RXFP2 mutations had significantly reduced BMD. No other apparent cause of osteoporosis was evident in these subjects, whose testosterone levels and gonadal function were normal. Expression analyses showed the presence of RXFP2 in human and mouse osteoblasts. Stimulation of these cells with INSL3 produced a dose- and time-dependent increase in cAMP and cell proliferation, confirming the functionality of the RXFP2/INSL3 receptor-ligand complex. Consistent with the human phenotype, bone histomorphometric and mu CT analyses of Rxfp2(-/-) mice showed decreased bone mass, mineralizing surface, bone formation, and osteoclast surface compared with wildtype littermates. Conclusions: This study suggests for he first time a role for INSL3/RXFP2 signaling in bone metabolism and links RXFP2 gene mutations with human osteoporosis.

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