Journal
JOURNAL OF BIOTECHNOLOGY
Volume 177, Issue -, Pages 67-73Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2014.02.013
Keywords
Laccase; Agitation; Aeration; Copper induction; P. ostreatus
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Funding
- DGAPA-UNAM [IN210911, IN201813]
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Mycelial growth and laccase production by Pleurotus ostreatus CP50 cultured in a 10-L mechanically agitated bioreactor were assessed through a 2(3) factorial experimental design. The main effects and interactions of three factors (agitation, aeration and copper induction) over five responses (mu, alpha(Lacc), beta(Lacc), maximal volumetric laccase activity and maximal biomass concentration) were analyzed. P. ostreatus growth was significantly improved when culturing was conducted with high agitation (5.9 kW/m(3) s) and aeration flow (0.5 vvm) rates. Under the experimental conditions evaluated, no evidence of hydrodynamic stress affecting fungal growth was observed. However, the high agitation and aeration conditions were detrimental for the growth-associated laccase production constant (alpha(Lacc)), leading to a very complex optimization of the process. The maximal laccase volumetric activity (1.2 and 3.8 U/ml for non-induced and copper-induced cultures, respectively) was observed when the culturing was performed at a low agitation rate (0.9 kW/m(3) s) and a high aeration flow rate (0.5 vvm). Laccase proteolysis may explain the complex interactions observed between agitation and aeration and the effects of these factors on the laccase volumetric activity observed in the cultures. (C) 2014 Elsevier B.V. All rights reserved.
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