Facile production of ZnS quantum dot nanoparticles by Saccharomyces cerevisiae MTCC 2918

Microbial synthesis of nanoparticles is a green route towards ecofriendly measures to overcome the toxicity and non-applicability of nanomaterials in clinical uses obtained by conventional physical and chemical approaches. Nanoparticles in the quantum regime have remarkable characteristics with excellent applicability in bioimaging. Yeasts have been commercially exploited for several industrial applications. ZnS nanoparticles as semiconductor quantum dots have mostly been synthesized by bacterial species. Here in, we have attempted to produce ZnS nanoparticles in quantum regime by Saccharomyces cerevisiae MTCC 2918 fungus and characterize its size and spectroscopic properties. Intracellular ZnS nanoparticles were produced by a facile procedure and freeze thaw extraction using 1 mM zinc sulfate. The ZnS nanoparticles showed surface plasmon resonance band at 302.57 nm. The ZnS nanoparticles were in low yield and in the size range of 30-40 nm. Powder XRD analysis revealed that the nanoparticles were in the sphalerite phase. Photoluminescence spectra excited at 280 nm and 325 nm revealed quantum confinement effects. This suggests that yeasts have inherent sulfate metabolizing systems and are capable fungal sources to assimilate sulfate. Further insights are required to identify the transport/reducing processes that may have caused the synthesis of ZnS nanoparticles such as an oxidoreductase enzyme-mediated mechanism. (C) 2013 Elsevier B.V. All rights reserved.