Journal
JOURNAL OF BIOTECHNOLOGY
Volume 169, Issue -, Pages 51-62Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2013.10.036
Keywords
Acremonium chrysogenum; Cephalosporin C; ELP/FRT recombination system; Inducible promoter; Targeted insertion of foreign DNA
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Funding
- Christian Doppler Society (Vienna, Austria)
- Sandoz GmbH (Kundl, Austria)
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Acremonium chrysogenum is the major producer of the p-lactam antibiotic cephalosporin C and therefore of great importance for the pharmaceutical industry. However, this filamentous fungus is known to reproduce solely by asexual means, shows only sporadic conidiospore production, and has gradual fragmentation of the vegetative mycelium into arthrospores. Due to these peculiar growth characteristics and life style, strain improvement by recombinant technologies is much more challenging than for other biotechnologically relevant fungi. Here, we describe several molecular tools for genetic engineering of A. chrysogenum, including a Delta Acku70 deletion strain for homologous recombination. No physiological or morphological changes occurred due to deletion of the ku70 gene or integration of the nat1 cassette in this recipient strain. We also used a xylose-inducible promoter from Sordaria macrospora (Smxyl) to demonstrate induction of the gfp reporter gene in A. chrysogenum. The Smxyl promoter was used for construction of a vector molecule to develop a one-step FLP/FRT recombination system in A. chrysogenum. This system was then used in the Delta Acku70 deletion strain to construct a marker-free recipient strain for targeted DNA insertion into genomic DNA. The applicability of our tools was demonstrated by construction of a marker-free transgenic strain, lacking any foreign genes. (C) 2013 Elsevier B.V. All rights reserved.
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