Journal
JOURNAL OF BIOTECHNOLOGY
Volume 188, Issue -, Pages 42-47Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2014.08.014
Keywords
Enantioselective resolution; Biotransformation; Halohydrin dehalogenase; (S)-2,3-dichloro-1-propanol; Biphasic system
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Funding
- National Basic Research Program of China (973 Program) [2011CB710800]
- National Natural Science Foundation of China [21176224]
- Key Project for Natural Science Foundation of Zhejiang Province [Z4080032]
- Research Fund for the Doctoral Program of Higher Education of China [2012331712004]
- Scientific Research Project of Education Department of Zhejiang Province [Y201121607]
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The enantioselective resolution of (R,S)-2,3-dichloro-1-propanol ((R,S)-DCP) to (S)-DCP by whole cells of a recombinant Escherichia coli expressing halohydrin dehalogenase (HHDH) activity was limited by product inhibition. To solve this problem to improve the productivity of (S)-DCP, an n-heptane-aqueous biphasic system was adopted in this work. The influential operational parameters including phase volumetric ratio, buffer pH and reaction temperature were optimized. Under the optimal reaction conditions, significant improvements of substrate concentration and biocatalyst productivity (375 mM and 7.64 mmol(S)-DCP g(-1) cell) were achieved in this n-heptane-aqueous biphasic system compared with aqueous single-phase system (150 mM and 2.97 mmol g(-1) cell). The scale-up biosynthesis of (S)-DCP was successfully performed in a 2-L stirred reactor, resulting in a 128.8 mM (S)-DCP with enantiomeric excess of 99.1% and average productivity of 2.07 g (S)-DCP L-1 h(-1), respectively. (C) 2014 Elsevier B.V. All rights reserved.
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