Journal
JOURNAL OF BIOTECHNOLOGY
Volume 188, Issue -, Pages 122-129Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2014.07.453
Keywords
Human neural stem cells; Differentiation; Induced pluripotent stem cells; Human embryonic stem cells; Neural rosette; Suspension culture
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Funding
- Arkansas Breast Cancer Research Program
- University of Arkansas for Medical Sciences Translational Research Institute (CTSA) [UL1TR000039]
- Women's giving circle at University of Arkansas
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Despite significant advances in commercially available media and kits and the differentiation approaches for human neural stem cell (NSC) generation, NSC production from the differentiation of human pluripotent stem cell (hPSC) is complicated by its time-consuming procedure, complex medium composition, and purification step. In this study, we developed a convenient and simplified NSC production protocol to meet the demand of NSC production. We demonstrated that NSCs can be generated efficiently without requirement of specific small molecules or embryoid body formation stage. Our experimental results suggest that a short suspension culture period may facilitate ectoderm lineage specification rather than endoderm or mesoderm lineage specification from hPSCs. The method developed in this study shortens the turnaround time of NSC production from both human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) differentiation. It provides a straightforward and useful strategy for generating NSCs that can benefit a wide range of research applications for human brain research. (C)2014 Elsevier B.V. All rights reserved.
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