Journal
JOURNAL OF BIOTECHNOLOGY
Volume 165, Issue 1, Pages 45-51Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2013.02.003
Keywords
Biodegradable resin; Biodiesel; mcl-PHA; Polyhydroxyalkanoate; Pseudomonas chlororaphis
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Funding
- European Commission [245084]
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A novel description of mcl-PHA biosynthesis by Ps. chlororaphis from tallow-based biodiesel as an inexpensive carbon feed stock is presented. Fermentation protocols, kinetic analysis, an efficient product recovery strategy, and product characterization are included. Maximum specific growth rates (mu(max.)) of 0.08 h(-1), 0.10 h(-1) and 0.13 h(-1), respectively, were achieved in three different fermentation set-ups. Volumetric productivity for mcl-PHA amounted to 0.071 g/L h, 0.094 g/L h and 0.138 g/L h, final intracellular PHA contents calculated from the sum of active biomass and PHA from 22.1 to 29.4 wt.-%, respectively. GC-FID analysis showed that the obtained biopolyester predominantly consists of 3-hydroxyoctanoate and 3-hydroxydecanoate, and, to a minor extent, 3-hydroxydodecanoate, 3-hydroxynonanoate, 3-hydroxyhexanoate, and 3-hydroxyheptanoate monomers. The overall distribution of the monomers remained similar, regardless to working volumes, biodiesel concentrations and pre-treatment of the inoculum. (C) 2013 Elsevier B. V. All rights reserved.
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