Journal
JOURNAL OF BIOTECHNOLOGY
Volume 168, Issue 2, Pages 130-134Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2013.03.016
Keywords
Solid state fermentation; Cereals; Fungi; Gamma-linolenic acid; beta-Carotene
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Funding
- Grant Agency of the Ministry of Education [VEGA 1/0975/12]
- Slovak Research and Development Agency, Slovak Republic [APVV-0662-11, APVV-0294-11]
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Four Mucor strains were tested for their ability to grow on four cereal substrates and enriched them with gamma-linolenic acid (GLA) and beta-carotene. M. circinelloides CCF-2617 as the best producer accumulated of both GLA and beta-carotene in high amounts during utilization of rye bran/spent malt grains (3: 1). The first growth phase was characterized by rapid GLA biosynthesis, while distinct beta-carotene formation was found in the stationary fungal growth. Therefore various cultivation conditions were tested in order to optimize the yield of either GLA or beta-carotene. The fungus grown on cereal substrate supplemented with glucose produced maximal 8.5 mg beta-carotene and 12.1 g GLA in 1 kg fermented substrate, respectively. On the other hand, the highest amount of GLA in the fermented substrate (24.2 g/kg) was achieved when 30% of sunflower oil was employed to the substrate. Interestingly, beta-carotene biosynthesis was completely inhibited when either whey or linseed oil were added to the substrate. (C) 2013 Elsevier B. V. All rights reserved.
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