Journal
JOURNAL OF BIOTECHNOLOGY
Volume 160, Issue 3-4, Pages 195-201Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2012.03.006
Keywords
Calorimetry; Heat flow; Saccharomyces cerevisiae; Specific growth rate; Fed-batch; Control
Categories
Ask authors/readers for more resources
The specific growth rate of a Saccharomyces cerevisiae strain with glucose as limiting C-source was estimated from the measured heat flow produced by the cells. For the cultivation a standard 30 l laboratory bioreactor was used, which was extended in such a way that heat balancing is possible. The feed rate was adjusted by a feedforward/feedback controller such that the specific growth rate was kept on the desired set-point value. On the basis of experimental investigations it was demonstrated that the specific growth rate can be controlled at a given set point value below the critical value to prevent the production of growth-inhibitory ethanol due to the Crabtree effect. With this control strategy high biomass concentrations of more than 110 g l(-1) can be obtained. (C) 2012 Elsevier B. V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available