Journal
JOURNAL OF BIOTECHNOLOGY
Volume 161, Issue 1, Pages 34-41Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2012.05.011
Keywords
Autophagy; Caspase-3/7; Bcl-2; Sodium butyrate; CHO cells; Beclin-1
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Funding
- World Class University program through the National Research Foundation of Korea (NRF)
- Ministry of Education, Science, and Technology (MEST) [R31-2008-000-10071-0]
- Converging Research Center Program through the NRF
- MEST [2009-0082276]
- Intelligent Synthetic Biology Center of Global Frontier Project
- MEST, Republic of Korea [2011-0031962]
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Previous research showed that co-down-regulation of caspase-3/7 in rCHO cells, unlike Bcl-2 overexpression, did not effectively block apoptotic cell death induced by 3 mM sodium butyrate (NaBu). Here, it is found that the control of autophagy is also related to this different response to NaBu treatment. With NaBu treatment, co-down-regulation of caspase-3/7 enhanced autophagy induction, whereas Bcl-2 overexpression delayed onset of autophagy induction in a Beclin-1 independent manner. The blockage of autophagy showed a detrimental effect on cell viability even in the Bcl-2 overexpressing cells, which suggests the importance of autophagy control for successful anti-cell death engineering of rCHO cells. (C) 2012 Published by Elsevier B.V.
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