Journal
JOURNAL OF BIOTECHNOLOGY
Volume 161, Issue 1, Pages 42-48Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2012.05.015
Keywords
Genome-scale metabolic model; Ketogulonicigenium vulgare; 2-keto-L-Gulonic acid; Sulfate metabolism; Vitamin C
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Funding
- National Natural Science Foundation of China [20836003]
- Program for New Century Excellent Talents in University [NCET-10-0456]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Program for Advanced Talents within Six Industries of Jiangsu Province [2011-NY033]
- State Key Laboratory of Bioreactor Engineering
- 111 Project [111-2-06]
- Fundamental Research Funds for the Central Universities [JUDCF10017]
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Ketogulonicigenium vulgare WSH001 is an industrial strain commonly used in the vitamin C producing industry. In order to acquire a comprehensive understanding of its physiological characteristics, a genome-scale metabolic model of K. vulgare WSH001, iWZ663, including 830 reactions, 649 metabolites, and 663 genes, was reconstructed by genome annotation and literature mining. This model was capable of predicting quantitatively the growth of K. vulgare under l-sorbose fermentation conditions and the results agreed well with experimental data. Furthermore, phenotypic features, such as the defect in sulfate metabolism hampering the syntheses of l-cysteine, l-methionine, coenzyme A (CoA), and glutathione, were investigated and provided an explanation for the poor growth of K. vulgare in monoculture. The model presented here provides a validated platform that can be used to understand and manipulate the phenotype of K. vulgare to further improve 2-KLG production efficiency. (C) 2012 Elsevier B.V. All rights reserved.
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