Journal
JOURNAL OF BIOTECHNOLOGY
Volume 152, Issue 4, Pages 144-146Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2010.07.031
Keywords
Polyhydroxyalkanoate; Ralstonia eutropha; Copolymer; Propionate
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [70216828]
- New Energy and Industrial Technology Development Organization (NEDO)
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Lipopolysaccharides free P[3-hydroxybutyrate (3HB)-co-3-hydroxyvalerate (3HV)] production was achieved using recombinant Corynebacterium glutamicum harboring polyhydroxyalkanoate (PHA) biosynthetic genes from Ralstonia eutropha. Cells grown on glucose with feeding of propionate as a precursor of 3HV unit accumulated 8-47 wt% of P(3HB-co-3HV). The 3HV fraction in the copolymer was varied from 0 to 28 mol% depending on the propionate concentrations. (C) 2010 Elsevier B.V. All rights reserved.
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